This study aims to evaluate the relationship between the level of various forms of interaction among its key players and students’ satisfaction in online setting. The level of interaction was measured following Moore’s model of interaction between student and tutor, among students, and of students with content (1973). The quantitative descriptive survey was used to conduct this study using a self-tailored questionnaire. The population of the study comprised all male and female students enrolled in Bachelors’ degree programs of the Department of Education in Spring 2018 and Fall 2018 at a virtual university in Pakistan (N=1049). All students enrolled in 4-years’ B.Ed. Elementary, 1.5 years B.Ed. Secondary, 2.5 years B.Ed. Elementary and M. Ed. were included as sample of the study. The overall level of interaction was slightly above the midpoint on the scale. As expected, the learner-content interaction was the highest and interaction among learners was the lowest. Significant correlation was found between the level of interaction and satisfaction with the online learning mode. The findings suggest that the institutions should take necessary measures to enhance and expand the three types of interaction presented by Moore and investigated in this study.